Generator

Part:BBa_I766670:Design

Designed by: Eric Chou   Group: iGEM07_UCSF   (2007-08-16)


YopH-EE under medium constitutive promotor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 720
    Illegal PstI site found at 2035
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2035
    Illegal NotI site found at 2594
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2577
    Illegal XhoI site found at 1675
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 720
    Illegal PstI site found at 2035
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 720
    Illegal PstI site found at 2035
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1225


Design Notes

Device was created using a method of combinatorial cloning using the type IIs restriction endonuclease AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method.



Source

conposite part

References